The expression and purification of WNK8 kinase have been described previously (Urano et al
The expression and purification of WNK8 kinase have been described previously (Urano et al., 2012). Phosphorylation mutations of RACK1A (S122D, T162E, S122D/T162E, and S122A/T162A) were generated using the QuikChange Lightning Site-Directed Mutagenesis Kit (Invitrogen). similar levels as those of RACK1S122A/T162A. However , although the steady-state level of the RACK1AS122A/T162Aprotein was similar to wild-type RACK1A protein,…
